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Aquatic Laser Fluorescence Analyzer (ALFA)

WET Labs' Aquatic Laser Fluorescence Analyzer (ALFA) provides spectrally and temporally resolved measurements of key bio-environmental variables in oceanic, coastal, and fresh waters.
WET Labs’ Aquatic Laser Fluorescence Analyzer (ALFA) provides spectrally and temporally resolved measurements of key bio-environmental variables in oceanic, coastal, and fresh waters. Operation modes allow underway shipboard measurements and discrete sample analysis for accurate assessments of pigment biomass, phytoplankton community structure and physiology.
 
Features
• Spectral deconvolution of overlapped fluorescence bands with Raman normalization
• Improved assessment of chlorophyll concentrations for estimating phytoplankton biomass
• Discrimination of 5 phycobiliprotein pigments for structural phytoplankton characterization
• Fluorescence assessment of chromophoric dissolved organic matter (CDOM) 
• Measures variable fluorescence, Fv/Fm, with correction for background CDOM fluorescence
• Instrument software allows real-time data analysis and display with GPS coordinates and time
 
 

 

Specifications

Excitation Light Source 405 nm and 510 nm TEC lasers
Spectral Emission Detection 400 – 800 nm, CCD array spectrometer
Variable Fluorescence Detection Auto ranging PMT and 12 bit waveform digitizer, 0.01 mg m-3 Chl a
Pulse Control Programmable/automatic control modes, 50 μs – 1000 μs, 0.1-30 Hz
Sampling Rate Variable integration time, 0.1 s – 10 s (5 s typical)
Operating Platform Windows
Power Requirements 100-240 V AC, 24 W
Operating Temperature 4 deg C to +40  deg C
Dimensions (L x W X H) 36 cm x 41 cm x 15 cm (14” x 16” X 6”)
Weight (in air) 5.4 kg (12 lbs)
Configuration Modes Continuous flow-through measurements or discrete sample analysis
Water Sample Connectors 1/4” quick connect fittings
Measurement Sample Volume ~1cm3
Flow Rate Up to 1L/min (continuous), or ~100mL/min (pumped)
Pump Integrated peristaltic
Housing Materials Powder coated aluminum case
Computer Interface USB connector
 

 

The list below includes (as applicable) the current product brochure, manual, and quick guide; software manual(s); and application notes.

Title Type Publication Date PDF File
AN101: Change to ALFA sampling bottle caps Application Notes Thursday, June 29, 2017 AN101-ALFA-Bottle-Cap.pdf
ALFA Datasheet Product Datasheet Tuesday, July 4, 2017 datasheet-ALFA.pdf
Underway sampling of marine inherent optical properties on the Tara Oceans expedition as a novel resource for ocean color satellite data product validation White Paper Wednesday, July 5, 2017 Werdell-et-al-2014_LO Methods-Inpress.pdf
Fluorescence, pigment and microscopic characterization of Bering Sea phytoplankton community structure and photosynthetic competency in the presence of a Cold Pool during summer White Paper Wednesday, July 5, 2017 ALF-BeringSea-2013-Research-Article.pdf
Analysis of spectral excitation for measurements of fluorescence constituents in natural waters White Paper Wednesday, July 5, 2017 ALF_spectral_excitation_Chekalyuk-Hafez_OpEx_v21_pp29255-68_2013.pdf
Aquatic Laser Fluorescence Analyzer: Field Evaluation in the Northern Gulf of Mexico White Paper Wednesday, July 5, 2017 Chekalyuk_et_al_2014_ALFA-GoM__oe-22-18-21641.pdf
Next Generation of Advanced Laser Fluorescence Technology for Characterization of Natural Aquatic Environments White Paper Wednesday, July 5, 2017 ALF_NOPP_FinalReport_Dec2013.pdf
Optical proxy for phytoplankton biomass in the absence of photophysiology: Rethinking the absorption line height White Paper Wednesday, July 5, 2017 Roesler-Barnard-2014_LO Methods-Inpress.pdf
The characteristics of particulate absorption, scattering and attenuation coefficients in the surface ocean; Contribution of the Tara Oceans expedition White Paper Wednesday, July 5, 2017 Boss-et-al-2014_LO Methods-Inpress.pdf
Advanced Laser Fluorometry (ALF): New Technology for Oceanography and Environmental Monitoring Scientific Poster Tuesday, July 4, 2017 AGU-Ocean-Sciences-2014.pdf
ALFA Instrument Introduction and Applications Scientific Poster Tuesday, July 4, 2017 ALFA_Instrument-Introduction-and-Applications.pdf
ALFA A New Instrument for Characterization of Natural Aquatic Environments Scientific Poster Tuesday, July 4, 2017 Barnard ALFA - Ocean-Sciences-2012-Research-Poster.pdf
ALFA Quick Start Product Quick Guide Tuesday, July 4, 2017 Quick-Start-ALFA.pdf

What should I use to lubricate bulkhead connectors?

WET Labs recommends keeping the connectors on your meter lubricated using a good quality silicone spray lubricant, such as 3M Silicone Spray or Loctite 8021 spray. These can be purchased at your local hardware store. Avoid silicone grease. It is messy and attracts contaminants.

Do not use WD-40. It will destroy connectors.